Receptors for and effects of insulin and IGF-I in rat glomerular mesangial cells

Journal Article · Tue Mar 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6871410

Arnqvist, H J; Ballermann, B J; King, G L ^[1]

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1. Harvard Medical School, Boston, MA (USA) Univ. of Linkoping (Sweden)

Receptors for and biological effects of insulin and insulin-like growth factor I (IGF-I) were studied in cultured rat renal mesangial cells. Specific binding of {sup 125}I-IGF was over 200-fold greater than the specific binding of {sup 125}I-insulin. Fifty percent inhibition of {sup 126}I-insulin binding was obtained with 8 {times} 10{sup {minus}9} M unlabeled insulin. For {sup 125}I-IGF-I, 50% inhibition required 1.8 {times} 10{sup {minus}9} M unlabeled IGF-I. {sup 125}I-IGF-I was also displaced by IGF-II and insulin but at 10- and 100-fold lower potencies, respectively, than IGF-I. Cross-linking of {sup 125}I-insulin and {sup 125}I-IGF-I to their receptors, using disuccinimidyl suberate (DSS), and identification of the receptor with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography showed a band with a molecular mass of 135 kDa, probably corresponding to the {alpha}-subunit of the insulin receptor and a major band with a molecular mass of 145 kDa for the {alpha}-subunit of the IGF-I receptor. Both insulin and IGF-I stimulated the incorporation of ({sup 3}H)thymidine into DNA. A half-maximal effect was obtained at 1.6 {times} 10{sup {minus}8} M for insulin and 1.2 {times} 10{sup {minus}9} M for IGF-I. No additive effect on DNA synthesis was observed. Insulin at 8 {times} 10{sup {minus}10} M increased the accumulation of ({sup 14}C)glucose in mesangial cells, whereas IGF-I was 10-fold less potent.

• OSTI ID: 6871410
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:3; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
GLOMERULI
GLUCOSE
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
MONOSACCHARIDES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
POLYMERIZATION
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RATS
REACTION KINETICS
RECEPTORS
RIBOSIDES
RODENTS
SACCHARIDES
SWINE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES