D-Glucosone and L-sorbosone, putative intermediates of L-ascorbic acid biosynthesis in detached bean and spinach leaves. [Phaseolus vulgaris L. ; Spinacia oleracea L]

Journal Article · Thu Nov 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.1496· OSTI ID:5841631

Saito, Kazumi; Nick, J A; Loewus, F A ^[1]

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1. Washington State Univ., Pullman (USA)

D-(6-{sup 14}C)Glucosone that had been prepared enzymically from D-(6-{sup 14}C)glucose was used to compare relative efficiencies of these two sugars for L-ascorbic acid (AA) biosynthesis in detached bean (Phaseolus vulgaris L., cv California small white) apices and 4-week-old spinach (Spinacia oleracea L., cv Giant Noble) leaves. At tracer concentration, {sup 14}C from glucosone was utilized by spinach leaves for AA biosynthesis much more effectively than glucose. Carbon-14 from (6-{sup 14}C)glucose underwent considerable redistribution during AA formation, whereas {sup 14}C from (6-{sup 14}C)glucosone remained almost totally in carbon 6 of AA. In other experiments with spinach leaves, L-(U-{sup 14}C)sorbosone was found to be equivalent to (6-{sup 14}C)glucose as a source of {sup 14}C for AA. In the presence of 0.1% D-glucosone, conversion of (6-{sup 14}C) glucose into labeled AA was greatly repressed. In a comparable experiment with L-sorbosone replacing D-glucosone, the effect was much less. The experiments described here give substance to the proposal that D-glucosone and L-sorbosone are putative intermediates in the conversion of D-glucose to AA in higher plants.

• OSTI ID: 5841631
• Journal Information: Plant Physiology; (USA), Vol. 94:3; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCORBIC ACID
BIOSYNTHESIS
PHASEOLUS
PHYSIOLOGY
SPINACH
CARBON 14 COMPOUNDS
GLUCOSE
METABOLISM
SORBOSE
TRACER TECHNIQUES
ALDEHYDES
CARBOHYDRATES
FOOD
HEXOSES
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SYNTHESIS
VEGETABLES
VITAMINS
550201* - Biochemistry- Tracer Techniques