Effect of heat stress on the pattern of protein synthesis in wheat endosperm

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5841290

Inwood, W; Bernardin, J ^[1]

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1. USDA, Albany, CA (USA)

The exposure of detached wheat heads (T. aestivum L. cv Cheyenne) to elevated temperatures resulted not only in the induction of a typical set of high and low molecular weight heat shock proteins (hsps), but also in a differential effect on the synthesis of wheat storage proteins in endosperm tissue when monitored by SDS PAGE of {sup 35}S-labeled polypeptides. The synthesis of hsps in the endosperm had a rapid onset, reached a maximum rate within the first 2 hours at 40{degree}C, and then steadily decreased during the next four hours. When heads were returned to 25{degree}C after 3 hours at 40{degree}C, hsp synthesis did not cease abruptly, but gradually declined over the next several hours. High molecular weight glutenin protein synthesis was drastically reduced with the same time course as heat shock protein synthesis was induced at 40{degree}C. Conversely, the synthesis of gliadin proteins remained at a high level at 40{degree}C. The synthesis rates for glutenin and gliadin proteins remained at low and high levels, respectively, for as long as the elevated temperature was maintained up to 7 hours.

• OSTI ID: 5841290
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CEREALS
DAYS LIVING RADIOISOTOPES
ENERGY
EVEN-ODD NUCLEI
GRAMINEAE
HEAT
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
MAGNOLIOPHYTA
NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
WHEAT