Characterization of the heat-shock response and its effect on development in Myxococcus xanthus
Thesis/Dissertation
·
OSTI ID:5099005
The heat-shock response of Myxococcus xanthus was investigated and characterized. When shifted from 28 to 40{degree}C, log-phase cells rapidly ceased growth, exhibited a 50% reduction in CFU, and initiated synthesis of heat-shock proteins (hsps). Heat-shocked vegetative M. xanthus labeled with ({sup 35}S)methionine produced 18 major hsps. The hsps, analyzed by SDS-polyacrylamide gel electrophoresis and fluorography were characterized with regard to molecular weight, subcellular fraction, and temperature required for expression. Heat-shocked starvation-induced fruiting cells and glycerol-induced sporulating cells were also analyzed and characterized. Thirteen hsps were detected in fruiting cells shifting from 28 to 40{degree}C while glycerol-induced cells produced 17 hsps after shift to 40{degree}C. The effect of heat-shock on M. xanthus was also investigated during both starvation-induced and glycerol-induced development. Tn5 mutants of M. xanthus were constructed and screened for vegetative and developmental defects.
- Research Organization:
- Rhode Island Univ., Kingston, RI (USA)
- OSTI ID:
- 5099005
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560200* -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENERGY
EVEN-ODD NUCLEI
HEAT
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
560200* -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENERGY
EVEN-ODD NUCLEI
HEAT
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES