Positron emission tomographic imaging of tumors using monoclonal antibodies

Technical Report · Sat Dec 01 04:00:00 EST 1990

OSTI ID:5841170

Zalutsky, M R

The overall objective for this research project is to develop methods for utilizing Positron Emission Tomography (PET) to increase the clinical potential of radiolabelled monoclonal antibodies (MAbs). By labeling MAbs with positron-emitting nuclides, it should be possible to quantitate the dynamics of their three-dimensional distribution in vivo. Our long term goals are to apply this approach to investigate the following: normal tissue toxicity; radiation dose to the tumor; and early tumor imaging. The research plans of this proposal include the following specific aims: optimize labeling of MAbs with fluorine 18, bromine 76 and bromine 75; label MAb Mel-14 (reactive against human gliomas and melanomas) and its Fab and F(ab{prime}){sub 2} fragments while retaining immunoreactivity; determine the distribution of Mel-14 in athymic mice bearing human gliomas; determine pharmacokinetics of Mel-14 in nonhuman primates. Experiments with another MAb, TP-1, and iodine 124 and 131 are also planned. 8 figs.

• Research Organization: Duke Univ. Medical Center, Durham, NC (USA). Dept. of Radiology
• Sponsoring Organization: DOE; USDOE, Washington, DC (USA)
• DOE Contract Number: FG05-89ER60789
• OSTI ID: 5841170
• Report Number(s): DOE/ER/60789-2; ON: DE91012937
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
560160 -- Radionuclide Effects
Kinetics
& Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BROMINE 75
BROMINE 76
BROMINE ISOTOPES
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
DISTRIBUTION
DOCUMENT TYPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
FLUORINE 18
FLUORINE ISOTOPES
GLIOMAS
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
IMMUNOLOGY
IMMUNOTHERAPY
INTERMEDIATE MASS NUCLEI
IODINE 124
IODINE 131
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
MAMMALS
MEDICINE
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
OPTIMIZATION
OSTEOSARCOMAS
POSITRON COMPUTED TOMOGRAPHY
PRIMATES
PROCESSING
PROGRESS REPORT
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOIMMUNOSCINTIGRAPHY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RADIONUCLIDE KINETICS
RADIOTHERAPY
RODENTS
SARCOMAS
SCINTISCANNING
SKELETAL DISEASES
THERAPEUTIC USES
THERAPY
TISSUE DISTRIBUTION
TOMOGRAPHY
TOXICITY
TRACER TECHNIQUES
USES
VERTEBRATES