Changes in regional plasma extravasation in rats following endotoxin infusion

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Microvasc. Res.; (United States)

DOI:https://doi.org/10.1016/0026-2862(87)90084-7· OSTI ID:5840522

van Lambalgen, A A; van den Bos, G C; Thijs, L G

Regional differences in plasma extravasation during endotoxin shock in rats and a possible relationship with changes in regional blood flow were studied with radioactive isotopes (/sup 125/I-HSA, 51Cr-labeled red blood cells, microspheres) in anesthetized rats (pentobarbital). Shock was induced by intravenous infusion of endotoxin (Eschericia coli; 10 mg X kg-1) for 60 min (starting at t = 0); at t = 120 min, the experiments were terminated. These rats (n = 8) were compared with time-matched control rats (n = 8). A third group (rats killed 7.5 min after injection of /sup 125/I-HSA, i.e., no extravasation; n = 8) served as baseline. The amount of plasma extravasated in 2 hr of endotoxin shock was significantly increased over control values in skin (by 67%), colon (88%), skeletal muscle (105%), stomach (230%), pancreas (300%), and diaphragm (1300%). Losses of /sup 125/I-HSA into intestinal lumen and peritoneal cavity had also increased over control values by 146 and 380%, respectively. Blood flow was compromised in most organs except heart and diaphragm. Extravasation when normalized for total plasma supply was correlated with total blood supply; the more the blood supply decreased, the higher the normalized extravasation. In the diaphragm, however, blood supply and plasma leakage increased together. Decreased blood supply and plasma extravasation may be related but they could also be simultaneously occurring independent phenomena with a common origin.

• Research Organization: Free Univ. Amsterdam, The Netherlands
• OSTI ID: 5840522
• Journal Information: Microvasc. Res.; (United States), Vol. 34:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDOTOXINS
INFUSION
HEMIC DISEASES
PATHOGENESIS
BLOOD FLOW
CHROMIUM 51
ERYTHROCYTES
ESCHERICHIA COLI
IODINE 125
MICROSPHERES
RATS
TRACER TECHNIQUES
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RODENTS
TOXIC MATERIALS
TOXINS
VERTEBRATES
550901* - Pathology- Tracer Techniques