Albumin extravasation rates in tissues of anesthetized and unanesthetized rats

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Appl. Physiol.; (United States)

OSTI ID:5702105

Renkin, E M; Joyner, W L; Gustafson-Sgro, M; Plopper, G; Sibley, L

Bovine serum albumin (BSA) labeled with /sup 131/I was injected intravenously in chronically prepared, unanesthetized rats and into pentobarbital-anesthetized rats that had received 2 ml 5% BSA to help sustain plasma volume. Initial uptake rates (clearances) in skin, skeletal muscles, diaphragm, and heart (left ventricle) were measured over 1 h. BSA labeled with /sup 125/I was injected terminally to correct for intravascular /sup 131/I-BSA. Observed clearances were in the following order in both groups of animals: heart much greater than diaphragm approximately equal to skin greater than resting skeletal muscles. Differences between unanesthetized and anesthetized animals were small and inconsistently directed. Our results suggest that the lower albumin clearances reported in the literature for anesthetized rats are not the result of their immobility or any direct effect of anesthesia on albumin transport in these tissues. The lower transport rates appear to result indirectly from changes produced by anesthesia and/or surgery in controllable parameters such as plasma volume and intravascular protein mass.

• Research Organization: Univ. of California, Davis (USA)
• OSTI ID: 5702105
• Journal Information: J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 66:5; ISSN JAPYA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANESTHESIA
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD PRESSURE
BODY
CARDIOVASCULAR SYSTEM
CLEARANCE
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
HEART
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MUSCLES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SKIN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES