Application of integral-equation theory to aqueous two-phase partitioning systems

Journal Article · Wed Sep 01 04:00:00 EDT 1993 · AIChE Journal (American Institute of Chemical Engineers); (United States)

DOI:https://doi.org/10.1002/aic.690390913· OSTI ID:5836374

Haynes, C A; Benitez, F J; Blanch, H W; Prausnitz, J M ^[1]

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1. Univ. of California, Berkeley, CA (United States)

A molecular-thermodynamic model is developed for representing thermodynamic properties of aqueous two-phase systems containing polymers, electrolytes, and proteins. The model is based on McMillan-Mayer solution theory and the generalized mean-spherical approximation to account for electrostatic forces between unlike ions. The Boublik-Mansoori equation of state for hard-sphere mixtures is coupled with the osmotic virial expansion truncated after the second-virial terms to account for short-range forces between molecules. Osmotic second virial coefficients are reported from low-angle laser-light scattering (LALLS) data for binary and ternary aqueous solutions containing polymers and proteins. Ion-polymer specific-interaction coefficients are determined from osmotic-pressure data for aqueous solutions containing a water-soluble polymer and an alkali chloride, phosphate or sulfate salt. When coupled with LALLS and osmotic-pressure data reported here, the model is used to predict liquid-liquid equilibria, protein partition coefficients, and electrostatic potentials between phases for both polymer-polymer and polymer-salt aqueous two-phase systems. For bovine serum albumin, lysozyme, and [alpha]-chymotrypsin, predicted partition coefficients are in excellent agreement with experiment.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5836374
• Journal Information: AIChE Journal (American Institute of Chemical Engineers); (United States), Journal Name: AIChE Journal (American Institute of Chemical Engineers); (United States) Vol. 39:9; ISSN AICEAC; ISSN 0001-1541
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
ALBUMINS
AQUEOUS SOLUTIONS
BINARY MIXTURES
BLOOD SERUM
DATA
DIFFUSION
DISPERSIONS
ELECTROLYTES
ELECTROSTATICS
ENZYMES
EQUILIBRIUM
EXPERIMENTAL DATA
FLUID MECHANICS
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
LASERS
LIGHT SCATTERING
LYSOZYME
MATHEMATICAL MODELS
MECHANICS
MIXTURES
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OSMOSIS
PARTITION
PHASE STUDIES
PHYSICAL PROPERTIES
POLYMERS
PROTEINS
SCATTERING
SOLUTIONS
THERMODYNAMIC PROPERTIES