Phase equilibria for aqueous protein/polyelectrolyte gel systems

Journal Article · Thu Aug 01 04:00:00 EDT 1996 · AIChE Journal

DOI:https://doi.org/10.1002/aic.690420823· OSTI ID:367870

Sassi, A P; Blanch, H W; Prausnitz, J M ^[1]

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1. Lawrence Berkeley Lab., CA (United States)

A molecular-thermodynamic analysis is directed toward predicting the partitioning of aqueous proteins into charged hydrogels. This analysis takes into account size exclusion by the network, electrostatic interactions, and the osmotic-pressure difference between a hydrogel and its surrounding solution. Electrostatic interactions in the polyelectrolyte gel can be described by Debye-Hueckel theory, or the Mean Spherical Approximation, or Katchalsky`s cell model for polyelectrolyte solutions. The cell model gives best agreement with experimental partition coefficients for cytochrome c. The quasi-electrostatic potential difference between a gel and its surrounding solution demonstrates how the electrostatic contribution to the protein partition coefficient depends on protein charge, gel-charge density, and solution ionic strength. Finally, a qualitative guide is presented for design of a polyelectrolyte gel such that it exhibits specified swelling and partitioning properties.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 367870
• Journal Information: AIChE Journal, Journal Name: AIChE Journal Journal Issue: 8 Vol. 42; ISSN AICEAC; ISSN 0001-1541
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AQUEOUS SOLUTIONS
CARRIERS
CHEMICAL ENGINEERING
CYTOCHROMES
DRUGS
ELECTROLYTES
ELECTROSTATICS
EQUILIBRIUM
GELS
MATHEMATICAL MODELS
PARTITION
PHASE STUDIES
PROTEINS
SEPARATION PROCESSES