Delineation of a previously unrecognized cis-acting element required for HLA class 2 gene expression

Journal Article · Fri Nov 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.22.10347· OSTI ID:5827254

Sugawara, M; Ponath, P D; Shin, Jaekyoon; Yang, Zhi; Strominger, J L ^[1]

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1. Harvard Univ., Cambridge, MA (United States)

The cis-acting DNA sequences that control expression of the HLA-DPA and HLA-DQB promoters have been investigated in detail. A set of recombinant plasmids containing 5{prime} promoter deletions as well as site-directed mutants of the DPA and DQB genes were fused to a reporter gene and transfected into human B and interferon {gamma} (IFN-{gamma})-inducible cells. A previously unrecognized cis element, which is essential both for transcription induced by IFN-{gamma} in several cell types and for constitutive class 2 expression in B cells, in addition to the well known X and Y boxes, has been defined. This sequence, which spans nucleotides {minus}107 to {minus}98 and {minus}146 to {minus}137 of the DPA and DQB promoters, respectively, has been called the J element. Some evidence for function of a fourth element, the S element, is also presented.

• OSTI ID: 5827254
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:22; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
DNA SEQUENCING
GENE REGULATION
GENE REPRESSORS
HELA CELLS
HISTOCOMPATIBILITY COMPLEX
MAMMALS
MAN
MUTAGENESIS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES