Differential distribution of AT sub 1 and AT sub 2 angiotensin II receptor subtypes in the rat brain during development

Journal Article · Sun Dec 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.24.11440· OSTI ID:5827082

Millan, M A; Catt, K J; Aghuilera, G ^[1]; Jacobowitz, D M ^[2]

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1. National Inst. of Child Health and Human Development, Bethesda, MD (United States)
2. National Inst. of Health, Bethesda, MD (United States)

Angiotensin II (AII) receptor subtypes were analyzed in the brains of adult and 2-week-old rats by in vitro autoradiography with {sup 125}I-labeled (Sar{sup 1},Ile{sup 8}) AII and competition studies with three AII antagonists: the nonpeptide antagonist, DuP 753, which is specific for AT{sub 1}receptors that mediate the calcium-inositol phospholipid signaling actions of AII; and nonpeptide (PD 123177) and peptide (CGP 42112A) antagonists that are selective for AT{sub 2} receptors of yet unknown function. In the adult rat brain, DuP 753 inhibited radioligand binding to the circumventricular organs and paraventricular nucleus but not to the lateral septum, subthalamic nucleus, and inferior olive. However, binding of {sup 125}I-labeled (Sar{sup 1}, Ile{sup 8}) AII in the latter regions was inhibited by the AT{sub 2} receptor antagonists PD 123177 and CGP 42112A. These areas showed similar displacement by the AT{sub 2} receptor subtype-specific antagonists in 2-week-old rats. These studies have demonstrated that presence of two AII receptor subtypes in the brain, one (AT{sub 1}) in areas related to regulation of blood pressure, water intake, and pituitary hormone secretion, and one (AT{sub 2}) whose function is not yet defined. The abundance and location of brain AT{sub 2} receptors in young animals, and the age-related changes in relative expression of the receptor subtypes, suggest that AII exerts specific actions according to the developmental stage of the central nervous system.

• OSTI ID: 5827082
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:24; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADULTS
AGE GROUPS
ANGIOTENSIN
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
GLOBULINS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIPIDS
MAMMALS
MEMBRANE PROTEINS
NEONATES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
TISSUE DISTRIBUTION
VASOCONSTRICTORS
VERTEBRATES