Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage

Journal Article · Wed Feb 01 04:00:00 EST 1984 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.81.3.781· OSTI ID:5825974

Tyrrell, R M

Nondividing (arrested) populations of primary human fibroblasts from normal individuals exposed to an intial dose (1.5 or 3 Jm/sup -2/) of far-UV (254 nm) radiation and then incubated in medium containing low (0.5%) serum develop enhanced resistance to inactivation of cloning efficiency by a second (challenge) dose of UV. The resistance develops within 2-4 days, after which there is a decline. Resistance develops to a higher degree and more rapidly (1-2 days) in cells derived from patients with the variant form of xeroderma pigmentosum. Excision-deficient cells from xeroderma pigmentosum complementation group A individuals also develop UV resistance after a lower (0.2 Jm/sup -2/) exposure to UV. Enhanced UV resistance does not develop in UV-irradiated cell populations incubated with the protein synthesis inhibitor cycloheximide (5 ..mu..M). These observations are consistent with the interpretation that exposure of human fibroblasts to low doses of UV induces synthesis of a protein involved in a metabolic pathway that transiently enhances the capacity of cells to repair potentially lethal damage resulting from a subsequent dose of UV.

• Research Organization: Swiss Institute for Experimental Cancer Research, Lausanne
• OSTI ID: 5825974
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81:3; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL PATHWAYS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DATA
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FAR ULTRAVIOLET RADIATION
FIBROBLASTS
FUNGICIDES
INFORMATION
IRRADIATION
LOW DOSE IRRADIATION
NUMERICAL DATA
PATIENTS
PESTICIDES
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
STIMULATION
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM