Exposure of nondividing populations of primary human fibroblasts to UV (254 nm) radiation induces a transient enhancement in capacity to repair potentially lethal cellular damage
Nondividing (arrested) populations of primary human fibroblasts from normal individuals exposed to an intial dose (1.5 or 3 Jm/sup -2/) of far-UV (254 nm) radiation and then incubated in medium containing low (0.5%) serum develop enhanced resistance to inactivation of cloning efficiency by a second (challenge) dose of UV. The resistance develops within 2-4 days, after which there is a decline. Resistance develops to a higher degree and more rapidly (1-2 days) in cells derived from patients with the variant form of xeroderma pigmentosum. Excision-deficient cells from xeroderma pigmentosum complementation group A individuals also develop UV resistance after a lower (0.2 Jm/sup -2/) exposure to UV. Enhanced UV resistance does not develop in UV-irradiated cell populations incubated with the protein synthesis inhibitor cycloheximide (5 ..mu..M). These observations are consistent with the interpretation that exposure of human fibroblasts to low doses of UV induces synthesis of a protein involved in a metabolic pathway that transiently enhances the capacity of cells to repair potentially lethal damage resulting from a subsequent dose of UV.
- Research Organization:
- Swiss Institute for Experimental Cancer Research, Lausanne
- OSTI ID:
- 5825974
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 81:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL REPAIR
STIMULATION
FIBROBLASTS
LOW DOSE IRRADIATION
RADIOSENSITIVITY
BIOLOGICAL PATHWAYS
CLONING
CYCLOHEXIMIDE
EXPERIMENTAL DATA
FAR ULTRAVIOLET RADIATION
PATIENTS
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
DATA
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
INFORMATION
IRRADIATION
NUMERICAL DATA
PESTICIDES
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SOMATIC CELLS
ULTRAVIOLET RADIATION
560121* - Radiation Effects on Cells- External Source- (-1987)