Genetic mapping in the lignin-degrading basidiomycete Phanerochaete chrysosporium
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:5825092
- Charles Univ., Prague (Czechoslovakia)
- Institute of Microbiology, Prague (Czechoslovakia)
The basidiomycete Phanerochaete chrysosporium is commonly used as a model organism in studies concerned with lignin biodegradation. In further investigations of the enzymology and regulation of ligninolytic activity as well as the construction of industrially applicable strains for biotechnological processing of lignin and lignocellulose, the genetics of this fungus is of great interest. A method of meiotic segregation analysis based on recombinant selection in the homothallic basidiomycete Phanerochaete chrysosporium was developed. Using this method, we were able to reveal linkage relationships and to estimate recombination frequencies between seven mutations to auxotrophy. We detected two linkage groups, the first containing four and the second three of the seven mapped mutations.
- OSTI ID:
- 5825092
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 57:1; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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