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Electrostimulation of rat callus cells and human lymphocytes in vitro

Journal Article · · J. Orth. Res.; (United States)
; ; ;

Asymmetrical pulsing low voltage current was supplied via electrodes to cultured rat fracture callus cells and human peripheral blood lymphocytes. The (/sup 3/H)thymidine incorporation of the callus cells and 5-(/sup 125/I)iodo-2'-deoxyuridine incorporation of the lymphocytes were determined. The growth pattern of callus cells (estimated by cellular density) did not respond to electrical stimulation. However, the uptake of (/sup 3/H)thymidine was increased at the early phase of cell proliferation and inhibited at later phases of proliferation. The (/sup 3/H)thymidine uptake of confluent callus cell cultures did not respond to electrical stimulation. Lymphocytes reacted in a similar way; stimulated cells took up more DNA precursor than control cells at the early phase of stimulation. During cell division, induced by the mitogens phytohemagglutinin and Concanavalin-A, the uptake of DNA precursor by stimulated cells was constantly inhibited. The results suggest that electrical stimuli affect the uptake mechanisms of cell membranes. The duality of the effect seems to be dependent on the cell cycle.

Research Organization:
Univ. of Turku, Finland
OSTI ID:
5824187
Journal Information:
J. Orth. Res.; (United States), Journal Name: J. Orth. Res.; (United States) Vol. 2:1; ISSN JORED
Country of Publication:
United States
Language:
English

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Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL CYCLE
CELL MEMBRANES
CELL PROLIFERATION
CONCANAVALIN
CONNECTIVE TISSUE CELLS
CURRENTS
DAYS LIVING RADIOISOTOPES
DEOXYURIDINE
DNA
DRUGS
ELECTRIC CURRENTS
ELECTRON CAPTURE RADIOISOTOPES
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANES
MITOGENS
MUCOPROTEINS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
STIMULATION
THYMIDINE
TRITIUM COMPOUNDS
UPTAKE
URACILS