Primary structure of the catalytic subunit of human DNA polymerase. delta. and chromosomal location of the gene

Journal Article · Sun Dec 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.24.11197· OSTI ID:5823149

Chung, D W; Davie, E W ^[1]; Zhang, Jian; Tan, Chengkeat; So, A G; Downey, K M ^[2]

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1. Univ. of Washington, Seattle (United States)
2. Univ. of Miami, FL (United States)

The catalytic subunit of human DNA polymerase {delta} has been cloned by PCR using poly (A){sup +}RNA from HepG2 cells and primers designed from the amino acid sequence of regions highly conserved between bovine and yeast DNA polymerase {delta}. The human cDNA was 3,443 nucleotides in length and coded for a polypeptide of 1,107 amino acids. The enzyme was 94% identical to bovine DNA polymerase {delta} and contained the numerous highly conserved regions previously observed in the bovine and yeast enzymes. The human enzyme also contained two putative zinc-finger domains in the carboxyl end of the molecule, as well as a putative nuclear localization signal at the amino-terminal end. The gene coding for human DNA polymerase {delta} was localized to chromosome 19.

• OSTI ID: 5823149
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
MOLECULAR STRUCTURE
HUMAN CHROMOSOME 19
GENETIC MAPPING
RECOMBINANT DNA
DNA SEQUENCING
DNA-CLONING
LIVER CELLS
MAN
ANIMAL CELLS
ANIMALS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
ENZYMES
HUMAN CHROMOSOMES
HYBRIDIZATION
MAMMALS
MAPPING
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry