Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.85.18.7018· OSTI ID:5822713

Alho, H; Fremeau, Jr, R T; Tiedge, H; Wilcox, J; Bovolin, P; Brosius, J; Roberts, J L; Costa, E

Diazepam binding inhibitor (DBI), an endogenous 10-kDa polypeptide was isolated from rat and human brain by monitoring displacement of radioactive diazepam bound to specific recognition sites in brain synaptic and mitochondrial membranes. The cellular location of DBI mRNA was studied in rat brain and selected peripheral tissues by in situ hybridization histochemistry with a /sup 35/S-labeled single-stranded complementary RNA probe. DBI mRNA was heterogeneously distributed in rat brain, with particularly high levels in the area postrema, the cerebellar cortex, and ependyma of the third ventricle. Intermediate levels were found in the olfactory bulb, pontine nuclei, inferior colliculi, arcuate nucleus, and pineal gland. Relatively low but significant levels of silver grains were observed overlying many mesencephalic and telencephalic areas that have previously been shown to contain numerous DBI-immunoreactive neurons and a high density of central benzodiazepine receptors. In situ hybridizations also revealed high levels of DBI mRNA in the posterior lobe of the pituitary gland, liver, and germinal center of the white pulp of spleen, all tissues that are rich in peripheral benzodiazepine binding sites. The tissue-specific pattern of DBI gene expression described here could be exploited to further understand the physiological function of DBI in the brain and periphery.

• Research Organization: Georgetown Univ. Medical Center, Washington, DC (USA)
• OSTI ID: 5822713
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Vol. 85:18
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
ENZYME INHIBITORS
GENE REGULATION
MESSENGER-RNA
TISSUE DISTRIBUTION
CYTOCHEMISTRY
HYBRIDIZATION
LIGANDS
PITUITARY GLAND
POLYPEPTIDES
RECEPTORS
SULFUR 35
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOISOTOPES
RNA
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques