Diazepam binding inhibitor gene expression: Location in brain and peripheral tissues of rate

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.85.18.7018· OSTI ID:5822713

Alho, H; Fremeau, Jr, R T; Tiedge, H; Wilcox, J; Bovolin, P; Brosius, J; Roberts, J L; Costa, E

Diazepam binding inhibitor (DBI), an endogenous 10-kDa polypeptide was isolated from rat and human brain by monitoring displacement of radioactive diazepam bound to specific recognition sites in brain synaptic and mitochondrial membranes. The cellular location of DBI mRNA was studied in rat brain and selected peripheral tissues by in situ hybridization histochemistry with a /sup 35/S-labeled single-stranded complementary RNA probe. DBI mRNA was heterogeneously distributed in rat brain, with particularly high levels in the area postrema, the cerebellar cortex, and ependyma of the third ventricle. Intermediate levels were found in the olfactory bulb, pontine nuclei, inferior colliculi, arcuate nucleus, and pineal gland. Relatively low but significant levels of silver grains were observed overlying many mesencephalic and telencephalic areas that have previously been shown to contain numerous DBI-immunoreactive neurons and a high density of central benzodiazepine receptors. In situ hybridizations also revealed high levels of DBI mRNA in the posterior lobe of the pituitary gland, liver, and germinal center of the white pulp of spleen, all tissues that are rich in peripheral benzodiazepine binding sites. The tissue-specific pattern of DBI gene expression described here could be exploited to further understand the physiological function of DBI in the brain and periphery.

• Research Organization: Georgetown Univ. Medical Center, Washington, DC (USA)
• OSTI ID: 5822713
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 85:18; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ENDOCRINE GLANDS
ENZYME INHIBITORS
EVEN-ODD NUCLEI
GENE REGULATION
GLANDS
HYBRIDIZATION
ISOTOPES
LIGANDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MESSENGER-RNA
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PITUITARY GLAND
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
SULFUR 35
SULFUR ISOTOPES
TISSUE DISTRIBUTION