Effect of endothelin-1 on calcium channel gating by agonists in vascular smooth muscle

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Cardiovasc. Pharmacol.; (United States)

DOI:https://doi.org/10.1097/00005344-198900135-00028· OSTI ID:5822346

Godfraind, T; Mennig, D; Morel, N; Wibo, M

Rat isolated aorta was more sensitive to the contractile effect of endothelin-1 (ET-1) when the endothelium was removed. ET-1 was more potent on mesenteric resistance arteries than on aorta. A threshold concentration of ET-1 (100 pM) enhanced the contractile responses of aortic rings to Bay K 8644 and clonidine, especially in the absence of endothelium. Potentiation of clonidine-evoked contraction was accompanied by an enhancement of /sup 45/Ca influx and was abolished by nifedipine. These actions of ET-1 (100 pM) could not be attributed to a decrease in membrane potential or in cAMP levels. ET-1 (100 pM) decreased cGMP in intact aortic rings, which could contribute to its actions in the presence of endothelium. Removal of endothelium reduced cGMP levels and these were not further decreased by ET-1. Since ET-1 exerted a pronounced potentiating effect in the absence of endothelium, it is likely that ET-1 modulates calcium channels by an additional mechanism, unrelated to cyclic nucleotides.

• Research Organization: Universite Catholique de Louvain, Brussels (Belgium)
• OSTI ID: 5822346
• Journal Information: J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States) Vol. 13 Suppl 5; ISSN JCPCD
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
AMP
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHLORIDES
CHLORINE COMPOUNDS
CONTRACTION
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENDOTHELIUM
EVEN-ODD NUCLEI
HALIDES
HALOGEN COMPOUNDS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
METALS
MUSCLES
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
TISSUES
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES