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Characterization of the human p53 gene promoter

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.9.5.2163· OSTI ID:5822335
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Transcriptional deregulation of the p53 gene may play an important part in the genesis of some tumors. The authors report here an accurate determination of the transcriptional start sites of the human p53 gene and show that the majority of p53 mRNA molecules do not contain a postulated stem-loop structure at their 5' ends. Recombinant plasmids of the human p53 promoter-leader region fused to the bacterial chloramphenicol acetyltransferase gene (cat) were constructed. After transfection into rodent or human cells, a 350-base-pair fragment spanning the promoter region conferred 4% of the CAT activity mediated by the simian virus 40 early promoter/enhancer. They monitored the efficiency with which 15 3' and 5' promoter deletion constructs initiated transcription. Their results show that an 85-base-pair fragment, previously thought to have resided in exon 1, is that is required for full promoter activity.

Research Organization:
Molecular Virology Lab., Imperial Cancer Research Fund, Lincoln's Inn Fields, London (GB); Imperial Cancer Research Fund Tumour Virus Group, Dept. of Pathology, Cambridge Univ., Tennis Court Road, Cambridge (GB)
OSTI ID:
5822335
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 9:5; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CARCINOMAS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CHLORAMPHENICOL
DISEASES
DNA
DRUGS
ENZYMES
GENE AMPLIFICATION
GENE REGULATION
GENE REPRESSORS
GENETIC MAPPING
HYBRIDIZATION
MAPPING
MESSENGER-RNA
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PLASMIDS
PROMOTERS
PROTEINS
RECOMBINANT DNA
RNA
TRANSCRIPTION
TRANSFERASES
TUMOR PROMOTERS