In vivo protein-DNA interactions at the. beta. -globin gene locus

Journal Article · Fri Nov 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.22.10188· OSTI ID:5822143

Ikuta, Tohru; Kan, Yuet Wai ^[1]

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1. Univ. of California, San Francisco (United States)

The authors have investigated in vivo protein-DNA interactions in the {beta}-globin gene locus by dimethyl sulfate (DMS) footprinting in K562 cells, which express {var epsilon}- and {gamma}-globin but not {beta}-globin. In the locus control region, hypersensitive site 2 (HS-2) exhibited footprints in several putative protein binding motifs. HS-3 was not footprinted. The {beta} promoter was also not footprinted, while extensive footprints were observed in the promoter of the active {gamma}-globin gene. No footprints were seen in the {sup A}{gamma} and {beta}3{prime} enhancers. With several motifs, additional protein interactions and alterations in binding patterns occurred with hemin induction. In HeLa cells, some footprints were observed in some of the motifs in HS-2, compatible with the finding that HS-2 has some enhancer function in HeLa cells, albeit much weaker than its activity in K562 cells. No footprint was seen in B lymphocytes. In vivo footprinting is a useful method for studying relevant protein-DNA interactions in erythroid cells.

• OSTI ID: 5822143
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:22; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA
DNA SEQUENCING
GENE REGULATION
GENE REPRESSORS
GENES
GLOBINS
HELA CELLS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METHYLATION
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS