Transcriptional role of a conserved GATA-1 site in the human. var epsilon. -globin gene promoter

Journal Article · Wed May 01 04:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.5.2558· OSTI ID:5118689

Gong, Qihui; Stern, J; Dean, A ^[1]

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1. National Inst. of Diabetes and Digestive and Kidney Diseases, Bethesda, MD (United States)

The {var epsilon}-globin gene is the first of the human {beta}-like globin genes to be expressed during development. The authors have analyzed protein-DNA interactions in the {var epsilon}-globin promoter region by DNase I footprinting and electrophoretic mobility shift experiments using nuclear extracts from K562 human erythroid cells and from nonerythroid HeLa cells. A restricted set of ubiquitous proteins, including Sp1, bound to regions of the promoter including the CACCC and CCAAT sites. Three interactions, at positions {minus}213, {minus}165, and +3 relative to the transcription start site, were erythroid specific and corresponded to binding of GATA-1, transcription factor highly restricted to the erythroid lineage. Interestingly, the GATA-1 site at {minus}165 has been conserved in the promoters of 10 mammalian embryonic globin genes. Point mutations demonstrate that GATA-1 binding to this site is necessary for interaction with an erythroid-specific enhancer but that in the absence of an enhancer, GATA-1 does not increase transcription.

• OSTI ID: 5118689
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:5; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CELL CONSTITUENTS
DNA-ASE
ELECTROPHORESIS
ENZYMES
ESTERASES
GENE MUTATIONS
GENE REGULATION
GENE REPRESSORS
GLOBINS
HELA CELLS
HYDROLASES
MAMMALS
MAN
MUTATIONS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLASMIDS
PRIMATES
PROTEINS
TRANSCRIPTION
VERTEBRATES