Complementary DNA sequencing: Expressed sequence tags and human genome project

Journal Article · Fri Jun 21 04:00:00 EDT 1991 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.2047873· OSTI ID:5820980

Adams, M D; Kelley, J M; Gocayne, J D; Dubnick, M; Wu, A; Olde, B; Moreno, R F; Kerlavage, A R; McCombie, W R; Venter, J C ^[1]; Polymeropoulos, M H; Xiao, Hong; Merril, C R ^[2]

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1. National Institutes of Health, Bethesda, MD (United States)
2. National Inst. of Mental Health, Washington, DC (United States)

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.

• OSTI ID: 5820980
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 252:5013; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ENZYMES
GENE AMPLIFICATION
GENETIC MAPPING
HYBRIDIZATION
MAMMALS
MAN
MAPPING
NERVOUS SYSTEM
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
RESEARCH PROGRAMS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES