2058 Expressed sequence tags (ESTs) from a human fetal lung cDNA library

Journal Article · Tue Nov 15 04:00:00 EST 1994 · Genomics

OSTI ID:250259

Kazunori, Sudo ^[1]; Chinen, Katsuya; Nakamura, Yusuke ^[2]

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1. Cancer Institute, Tokyo (Japan)
2. Mitsubishi Yuka BCL Co. Ltd., Tokyo (Japan)

ESTs (expressed sequence tags) provide complementary resources for structural and functional analyses of the human genome. The authors have performed single-pass sequencing of 2058 randomly selected, directionally cloned cDNAs isolated from a fetal-lung cDNA library constructed with oligo (dT) primers. Computer analyses of the 5{prime}-end sequences revealed that 60.4% of the clones were considered to be identical to previously reported human genes or ESTs; 9.0% of them showed significant homology to known genes in human, other mammals, or lower organisms; 30.6% showed no homology to any genes or DNA sequences in the public database. These data and reagents will be useful for future investigations of gene expression during prenatal development of human lung. 11 refs., 1 fig., 2 tabs.

• OSTI ID: 250259
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 24; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOMES
LUNGS
ONTOGENESIS