In vivo biodistribution of a radiolabeled blood substitute: sup 99m Tc-labeled liposome-encapsulated hemoglobin in an anesthetized rabbit
- Naval Research Lab., Washington, DC (United States)
- Univ. of Texas, San Antonio (United States)
Liposome-encapsulated hemoglobin (LEH) is an erythrocyte substitute that is a potential resuscitative fluid for the in vivo delivery of oxygen. The authors had noninvasively imaged radiolabeled LEH in vivo with technetium-99m ({sup 99m}Tc) to study the biodistribution in an anesthetized rabbit. Rabbits were infused with 30 ml of LEH and imaged with a {gamma} camera continuously for 2 hr. At 20 hr postinfusion, the animals were imaged again and sacrificed; the organs were weighed and their radioactivity was determined for autopsy organ distribution. Organ uptake from the images was corrected for organ-associated blood pool, which was determined by infusion of {sup 99m}Tc-labeled rabbit erythrocytes. Blood pool and decay-corrected biodistribution data reveal the kinetics of LEH distribution. Image biodistribution data was also validated at 20 hr by tissue sampling. At 20 hr postinfusion, autopsy biodistribution data reveals approximately 42.6% of the total counts remaining in the blood, 15.4% in the liver, 18.1% in spleen, 3.2% in the lungs, 2.4% in muscle, 1.6% in urine, and trace levels in the kidney, brain, and heart (<1%). There is no evidence of hemoglobin release from LEH or kidney dysfunction at any time over the course of the study.
- OSTI ID:
- 5820684
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:23; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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BLOOD SUBSTITUTES
DYNAMIC FUNCTION STUDIES
ERYTHROCYTES
TISSUE DISTRIBUTION
BLOOD
HEMOGLOBIN
ISOMERIC NUCLEI
LIPOSOMES
LIVER
LUNGS
MUSCLES
RABBITS
SPLEEN
TECHNETIUM 99
URINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
GLANDS
GLOBINS
HEMATOLOGIC AGENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
TECHNETIUM ISOTOPES
VERTEBRATES
WASTES
YEARS LIVING RADIOI
550601* - Medicine- Unsealed Radionuclides in Diagnostics