Spectroscopic study of lanthanide complexes with nucleosides and nucleotides

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5817252

Rudman, A

The terbium ethylenediaminotetraacetic acid (EDTA) complex exhibits stability over a wide pH range, is highly soluble in aqueous solutions, and has stoichiometry limited to 1:1:1 complexes. The use of ternary Tb(EDTA) complexes yields a system with a well defined stoichiometry and permits the comparison of spectra with solution phase structures. It has been shown that these ternary complexes exhibit all of the photophysical properties normally associated with Tb(III) nucleic acid complexes. The excitation and absorption spectra of numerous Tb(EDTA) nucleosides and nucleotides has shown that the metal ion is excited by direct excitation rather than from energy transfer through the ligands. The 304 nanometer absorption band associated with these Tb(EDTA) complexes, has been shown to be sensitized in the presence of guanosine and guanosine monophosphate. The mechanism associated with this hypersensitivity appears to require that the Tb(III) ion experience a polymeric environment. Finally both the hydroxyl group at the C-2' position on the ribose, and the purine and pyrimidine base residues have been shown to actively bind under basic pH conditions.

• Research Organization: Seton Hall Univ., South Orange, NJ (USA)
• OSTI ID: 5817252
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201* -- Chemical & Physicochemical Properties
400301 -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
400500 -- Photochemistry
AMINO ACIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMISTRY
COMPLEXES
EDTA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMISTRY
RARE EARTH COMPLEXES
TERBIUM COMPLEXES