Comparison of growth-induced resorption and denervation-induced resorption on the release of (/sup 3/H)tetracycline, /sup 45/calcium, and (/sup 3/H)collagen from whole bones of growing rats

Journal Article · Sat Jan 01 04:00:00 EST 1983 · J. Orth. Res.; (United States)

DOI:https://doi.org/10.1002/jor.1100010107· OSTI ID:5808214

Klein, L; Heiple, K G; Stromberg, B V

The major effect of immobilization during growth is a smaller bone mass induced by either an increased bone resorption or a decreased bone formation. Using a method of analyzing radioisotopic loss of (/sup 3/H)tetracycline and (/sup 3/H)collagen from bone prelabeled in vivo, we compared the amount of bone resorption due to immobilization with bone resorption induced by growth. One hind limb was denervated in growing male rats, 6 weeks of age, that had been chronically prelabeled with (/sup 3/H)tetracycline, /sup 45/calcium, and (/sup 3/H)proline. The total radioactivity of the whole femur and tibia/fibula from the denervated limb was compared with that from bones of the control limb at 0, 1, 2, 4, and 8 weeks after denervation. The effect of growth on bone formation was measured by net increases in bone length, volume, and mass of matrix and mineral. Experimental bones had a significantly smaller volume and mass. Bone resorption was much greater during growth modeling than during denervation. The additional bone resorption induced by denervation was a small fraction (one-fourth) of the resorption induced by growth. Denervation during growth resulted in less bone being formed due to a smaller gain in matrix and mineral mass as a result of a reduction in bone formation.

• Research Organization: Case Western Reserve Univ. School of Medicine, Cleveland, OH
• OSTI ID: 5808214
• Journal Information: J. Orth. Res.; (United States), Journal Name: J. Orth. Res.; (United States) Vol. 1:1; ISSN JORED
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
AMINES
AMINO ACIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
COLLAGEN
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VIVO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
NERVES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIOISOTOPES
RATS
RODENTS
SCLEROPROTEINS
SKELETON
TETRACYCLINES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES