Tumor necrosis factor alpha and interleukin-1 stimulate bone resorption in vivo as measured by urinary ( sup 3 H)tetracycline excretion from prelabeled mice

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Journal of Bone and Mineral Research; (USA)

OSTI ID:5517021

Koenig, A M; Muehlbauer, R C.F.; Fleisch, H ^[1]

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1. Univ. of Berne (Switzerland)

Tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1) have been shown to stimulate bone resorption in vitro. We have now investigated whether these cytokines also cause a similar action when administered in vivo. This was made possible by the adaptation of a newly developed technique that enables the continual assessment of bone resorption in vivo in mice by measuring urinary excretion of {sup 3}H from ({sup 3}H)tetracycline-prelabeled animals. Experiments using maneuvers known to influence bone resorption, such as a change in dietary calcium or administration of parathyroid hormone or dichloromethylenebisphosphonate, indicate that the technique is reliable and sensitive in mice. Daily intravenous administration of either recombinant human or recombinant murine TNF-alpha, as well as subcutaneous administration of recombinant human IL-1 alpha, were found to stimulate bone resorption in a dose-dependent manner. The effect was maximal within 2 days. Thus, exogenous TNF-alpha and IL-1 alpha can stimulate bone resorption in vivo, suggesting that these cytokines may also exert a systemic effect on bone.

• OSTI ID: 5517021
• Journal Information: Journal of Bone and Mineral Research; (USA), Journal Name: Journal of Bone and Mineral Research; (USA) Vol. 3:6; ISSN JBMRE; ISSN 0884-0431
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM
CLEARANCE
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
EXCRETION
GROWTH FACTORS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LYMPHOKINES
MAMMALS
METABOLISM
METALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SKELETON
SORPTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES