Multi-hit Weigle-reactivation of uv-phage lambda in a fraction of complexes with uv-E. coli K12

Conference · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5808099

Fluke, D J; Bronner, C E; Pollard, E C

While W-reactivation plaque survival curves conform reasonably well to dose-modifying factors through most of the lower range in plaque survival, substantial departures from a DMF description occur in the first decade of plaque survival. For 265-nm uv lambda in 265-nm uv E. coli AB1157 we have reported results consistent with 5 to 7 hits repaired in about 15% of complexes at maximum, with single-hit plaque survival at the same D/sub 0/ in the other 85%. Subsequent extension of these results over five decades of plaque survival show, at maximum W-reactivation, a 5-hit repair in 19% of the complexes, with a consistent D/sub 0/ throughout, about 13.4 J/m/sup 2/. Graded induction of the cells primarily changes the fraction of complexes giving the W-reactivation, and the maximum fraction is substantially independent of cell growth stage. At maximum W-reactivation, induced cells incubated post-uv showed some decline in the number of hits repairable, rather more decline in the fraction of complexes giving the W-reactivation, while holding fairly steady in D/sub 0/. The further studies also indicate that the process conforms very well to multi-hit target theory, although two other shouldered-curve models give fairly similar numerical results. 3 figures, 2 tables.

• Research Organization: Duke Univ., Durham, NC (USA). Dept. of Zoology
• DOE Contract Number: AS05-76EV03631
• OSTI ID: 5808099
• Report Number(s): CONF-830220-2; ON: DE85009645
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EXPERIMENTAL DATA
HOST-CELL REACTIVATION
INFORMATION
MICROORGANISMS
NUMERICAL DATA
PARASITES
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SURVIVAL CURVES
ULTRAVIOLET RADIATION
VIRUSES