Mitogen-stimulated phospholipid synthesis in normal and immune-deficient human B cells

Journal Article · Tue Apr 15 04:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:5806025

Chien, M M; Yokoyama, W M; Ashman, R F

Eight patients with common variable panhypogammaglobulinemia were shown in the in vitro Ig biosynthesis assay to have defective B cell responses to pokeweed mitogen (PWM). Phospholipid synthesis was assessed in the B cell plus monocyte fraction (MB) and irradiated T cells (T*) of patients and paired normal controls. Cell populations were studied separately and in the four possible combinations (1:1), with and without PWM, to reveal the effect of cell interactions. At 16 to 20 hr the mean stimulation index (SI) +/- standard error for MB cells alone was 1.01 +/- 0.02 for eight patients and 0.99 +/- 0.02 for the paired normals; the T* cell SI was 1.25 +/- 0.04 for patients and 1.28 +/- 0.05 for normals. Combinations of normal MB cells with normal T* cells showed significantly higher SI when compared with the combinations of normal MB cells with patient T* cells (p less than 0.005). However, the combination of patient MB cells with patient T* cells and the combination of patient MB cells with normal T* cells were not significantly different in SI (0.05 less than p less than 0.1). Isolation of patient and normal B cells, T* cells, and monocytes after the choline pulse showed that patient B cells gave a higher SI with normal T* help than with patient T* help. Of greatest interest is the finding that patient B cells that were defective in PWM-stimulated Ig production nevertheless showed a phospholipid synthesis response to PWM in the normal range, suggesting that the maturation defect in these B cells occurs later than the phospholipid synthesis acceleration step, or on a different pathway.

• Research Organization: Univ. of Iowa College of Medicine, Iowa City
• OSTI ID: 5806025
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 8; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
ESTERS
GLOBULINS
IMMUNOGLOBULINS
IRRADIATION
LEUKOCYTES
LIPIDS
LYMPHOCYTES
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PATIENTS
PHOSPHOLIPIDS
PROTEINS
SOMATIC CELLS
SYNTHESIS