Requirements of glycerol and fatty acid for triglyceride synthesis and ketogenesis by hepatocytes from normal and triiodothyronine-treated rats

Journal Article · Fri Nov 15 04:00:00 EST 1985 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(85)91906-0· OSTI ID:5805896

Olubadewo, J O; Heimberg, M

Hepatocytes from T3-treated rats synthesized less triglyceride and more ketone bodies from (1-/sup 14/C)oleate at all concentrations from 0-2 mM, than did hepatocytes from euthyroid animals; addition of 1.0 mM glycerol increased triglyceride synthesis and reduced ketogenesis in hepatocytes from T3-treated rats to the rates observed in euthyroid hepatocytes in the absence of added glycerol. Glycerol did not alter triglyceride synthesis, but reduced ketogenesis genesis by euthyroid hepatocytes. It is probable from these and other data that, in the hyperthyroid rat, glycero-3-P, and not fatty acid, is rate limiting for synthesis of triglyceride, and, secondarily for reducing rates of ketogenesis in the hepatocyte.

• OSTI ID: 5805896
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMAL CELLS
ANIMALS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ESTERS
GLANDS
GLYCEROL
HORMONES
HYDROXY COMPOUNDS
HYPERTHYROIDISM
IN VITRO
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LIPIDS
LIVER
LIVER CELLS
MAMMALS
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
THYROID HORMONES
TRACER TECHNIQUES
TRIGLYCERIDES
TRIIODOTHYRONINE
VERTEBRATES