Formation and repair of furocoumarin-DNA photoadducts in mammalian chromatin

Thesis/Dissertation · Sat Jan 01 00:00:00 EST 1983

OSTI ID:5804920

Zolan, M E

DNA damage and repair in response to ultraviolet light (UV) or furocoumarin photoadducts were studied in cultured primate cells. First, the sensitivity to digestion by the enzyme staphylococcal nuclease (SN) of repair patches synthesized in response to damage by UV or the angular furocoumarin angelicin was examined in human cells. Second, excision repair was examined in the 172 base pair highly repeated alpha DNA sequence in African green monkey cells. Excision repair after treatment of cells with UV was the same in alpha DNA as in the bulk of the genome. However repair of furocoumarin photoadducts was only about 30% as great in alpha DNA as in the bulk of the genome, even through the initial amount of damage, the time course of excision repair, and the excision repair patch size were all indistinguishable for the two DNA species.

• Research Organization: Stanford Univ., CA (USA)
• OSTI ID: 5804920
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL RADIATION EFFECTS
DNA ADDUCTS
BIOLOGICAL REPAIR
RADIOINDUCTION
ANIMAL CELLS
CHROMATIN
COUMARINS
NUCLEASES
ULTRAVIOLET RADIATION
ADDUCTS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HYDROLASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
560121* - Radiation Effects on Cells- External Source- (-1987)