Deficient repair of chemical adducts in alpha DNA of monkey cells
Researchers have examined excision repair of DNA damage in the highly repeated alpha DNA sequence of cultured African green monkey cells. Irradiation of cells with 254 nm ultraviolet light resulted in the same frequency of pyrimidine dimers in alpha DNA and the bulk of the DNA. The rate and extent of pyrimidine dimer removal, as judged by measurement of repair synthesis, was also similar for alpha DNA and bulk DNA. In cells treated with furocoumarins and long-wave-length ultraviolet light, however, repair synthesis in alpha DNA was only 30% of that in bulk DNA, although it followed the same time course. Researchers found that this reduced repair was not caused by different initial amounts of furocoumarin damage or by different sizes of repair patches, as researchers found these to be similar in the two DNA species. Direct quantification demonstrated that fewer furocoumarin adducts were removed from alpha DNA than from bulk DNA. In cells treated with another chemical DNA-damaging agent, N-acetoxy-2-acetylaminofluorene, repair synthesis in alpha DNA was 60% of that in bulk DNA. These results show that the repair of different kinds of DNA damage can be affected to different extents by some property of this tandemly repeated heterochromatic DNA. To our knowledge, this is the first demonstration in primate cells of differential repair of cellular DNA sequences.
- Research Organization:
- Department of Biological Sciences, Stanford University, California
- OSTI ID:
- 6879004
- Journal Information:
- Cell; (United States), Vol. 28:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
ADDUCTS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
COMPARATIVE EVALUATIONS
DIMERS
FLUORENE
MEASURING METHODS
PSORALEN
PYRIMIDINES
QUANTITY RATIO
ULTRAVIOLET RADIATION
ANTICOAGULANTS
AROMATICS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CONDENSED AROMATICS
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
KINETICS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
560121* - Radiation Effects on Cells- External Source- (-1987)
560111 - Radiation Effects on Biochemicals- In Vitro- (-1987)