Combination of high-performance liquid chromatography and radioimmunoassay for the measurement of urodilatin and. alpha. -hANP in the urine of healthy males

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90380-T· OSTI ID:5803804

Solc, J; Bauer, K; Timnik, A; Doehlemann, C; Strom, T M; Weil, J ^[1]; Solcova, A

---

1. Univ. of Munich, Muenchen (West Germany)

Urodilatin (ANP-(95-126)), a natriuretic peptide in urine, and {alpha}-hANP (ANP-(99-126)) are crossreactive in the radioimmunoassay of {alpha}-hANP (ANP-RIA). The authors therefore developed a method to separate physiological amounts of urodilatin and {alpha}-hANP in urine by high-performance liquid chromatography followed by ANP-RIA of the separated fractions. They studied urine samples of 10 healthy adult males with a plasma {alpha}-hANP level of 41 {plus minus} 21 pg/ml (mean {plus minus} SD) and a total urinary ANP-RIA reactivity of 40 {plus minus} 21 pg/ml. In all urine samples they found three peaks of ANP-RIA reactivity, the first one coeluting with synthetic urodilatin, the second one with the retention time of {alpha}-hANP and a late eluting ANP-RIA-reactive peak, possibly containing degradation products. The ratio of urodilatin/{alpha}-hANP was 0.77 {plus minus} 0.17.

• OSTI ID: 5803804
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 48:25; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
ORGANIC COMPOUNDS
PEPTIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES