Highly sensitive radioimmunoassay of atrial natriuretic peptide (ANP) in human plasma and urine
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
A highly sensitive radioimmunoassay has been established for measurement of human plasma and urine concentrations of atrial natriuretic peptide (ANP) and requires no extraction or concentration process. An antiserum was prepared from rabbits immunized with ..cap alpha..-human ANP (..cap alpha..-hANP) coupled with bovine-thyroglobulin. The sensitivity of this method was 0.2 pg/tube of synthetic ..cap alpha..-hANP utilized as authentic standard. Recovery of ..cap alpha..-hANP spiked to plasma and urine was 97.7 +/- 15.4% and 97.1 +/- 9.5% (mean +/- SD), respectively. Plasma and urinary ANP concentrations versus assay data showed satisfactory linearity. In 124 health subjects, the plasma ANP concentration was 31.7 +/- 12.0 pg/ml. Two different molecular forms of ANP in plasma and a single form in urine were found by gel permeation chromatography.
- Research Organization:
- Kitasato Univ. School of Medicine, Kanagawa, Japan
- OSTI ID:
- 5009235
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Combination of high-performance liquid chromatography and radioimmunoassay for the measurement of urodilatin and. alpha. -hANP in the urine of healthy males
Sensitive radioimmunoassay of. cap alpha. human atrial natriuretic polypeptide using monoclonal antibody recognizing human form ring structure
Relationship in humans between atrial natriuretic peptide and arginine vasopressin during dehydration
Journal Article
·
Mon Dec 31 23:00:00 EST 1990
· Life Sciences; (USA)
·
OSTI ID:5803804
Sensitive radioimmunoassay of. cap alpha. human atrial natriuretic polypeptide using monoclonal antibody recognizing human form ring structure
Journal Article
·
Thu Dec 31 23:00:00 EST 1987
· Life Sci.; (United States)
·
OSTI ID:6412379
Relationship in humans between atrial natriuretic peptide and arginine vasopressin during dehydration
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5318809
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CATTLE
CHROMATOGRAPHY
DATA
DOMESTIC ANIMALS
EXPERIMENTAL DATA
GEL PERMEATION CHROMATOGRAPHY
GLOBULINS
IMMUNE SERUMS
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MOLECULAR STRUCTURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PATIENTS
PEPTIDES
POLYPEPTIDES
PROTEINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RUMINANTS
SENSITIVITY
SEPARATION PROCESSES
THYROGLOBULIN
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CATTLE
CHROMATOGRAPHY
DATA
DOMESTIC ANIMALS
EXPERIMENTAL DATA
GEL PERMEATION CHROMATOGRAPHY
GLOBULINS
IMMUNE SERUMS
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MOLECULAR STRUCTURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PATIENTS
PEPTIDES
POLYPEPTIDES
PROTEINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RUMINANTS
SENSITIVITY
SEPARATION PROCESSES
THYROGLOBULIN
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES