Reverse-phase high-performance liquid chromatography of nerve growth factor receptor-like proteins identified with monoclonal antibodies

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Journal of Neuroscience Research; (USA)

DOI:https://doi.org/10.1002/jnr.490270423· OSTI ID:5803562

Shan, D E; Beck, C E; Werrbach-Perez, K; Perez-Polo, J R ^[1]

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1. Univ. of Texas Medical Branch, Galveston (USA)

Human neuroblastoma SK-N-SH-SY5Y (SY5Y) and rat pheochromocytoma PC12 cells are model cell lines used in the study of nerve growth factor (NGF) effect. The effects of NGF are initiated by binding to cell surface receptors (NGFR). The amino acid sequence for NGFR has been deduced based on the identification of a single gene for NGFR. However, there are two kinds of NGF binding activities and several reported molecular weights of NGFR. We report here on the demonstration of NGFR-like proteins from PC12 and SY5Y cells by sequential lectin chromatography, reverse-phase HPLC, and SDS-PAGE analysis of immunoprecipitates obtained with NGFR-specific monoclonal antibodies. For both human and rodent NGFR, there was a tendency for the higher molecular-weight species of NGFR-like proteins to be eluted in more hydrophobic fractions. Also, the expression of different species of NGFR could be modified by treatment with retinoic acid (RA). These results are consistent with the hypothesis that the different molecular species of NGFR may result from the generation of a truncated form of NGFR, the presence of sugar residues on the NGFR protein, dimer formation between NGFR, or the association of NGFR with a receptor-associated protein.

• OSTI ID: 5803562
• Journal Information: Journal of Neuroscience Research; (USA), Journal Name: Journal of Neuroscience Research; (USA) Vol. 27:4; ISSN JNRED; ISSN 0360-4012
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CHROMATOGRAPHY
ELECTROPHORESIS
GROWTH FACTORS
KINETICS
LECTINS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NERVES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SEPARATION PROCESSES
TUMOR CELLS
VERTEBRATES