Studies on nerve growth and repair
Thesis/Dissertation
·
OSTI ID:5177536
The transition of nerve cell from a dividing undifferentiated cell to a morphologically differentiated nondividing cell was studied. Pheochromocytoma (PC12) cells respond to nerve growth factor (NGF) by extending electrically excitable neurites. Yet by labeling these cells with /sup 3/H-thymidine and examining them autoradiographically, evidence for continued DNA replication in neurite bearing neurons was found. NGF receptors of two classes are expressed on PC12 cells and other NGF dependent neurons. By labeling differentiated PC12s in culture with /sup 125/I-NGF and examining them autoradiographically both receptor classes were found evenly distributed on the growth cones, neurites and cell bodies. Cytoskeletal associated receptors were found in these areas as well. The purification and identification of a soluble, extracellular protein of 37,000 molecular weight whose synthesis and accumulation is increased after injury of a rat sciatic nerve is described.
- Research Organization:
- Stanford Univ., CA (USA)
- OSTI ID:
- 5177536
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nerve growth factor-treated, neurite-bearing PC12 cells continue to synthesize DNA
Biochemical and biological properties of the nerve growth factor receptor
Binding and internalization of nerve growth factor by PC12 cells
Journal Article
·
Thu Jan 31 23:00:00 EST 1985
· J. Neurosci.; (United States)
·
OSTI ID:5554243
Biochemical and biological properties of the nerve growth factor receptor
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:5188073
Binding and internalization of nerve growth factor by PC12 cells
Thesis/Dissertation
·
Wed Dec 31 23:00:00 EST 1986
·
OSTI ID:7188077
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BIOELECTRICITY
BIOLOGICAL ACCUMULATION
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL DIFFERENTIATION
DATA
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
INFORMATION
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NERVE CELLS
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURIFICATION
PYRIMIDINES
RADIOISOTOPES
RATS
RECEPTORS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SCIATIC NERVE
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BIOELECTRICITY
BIOLOGICAL ACCUMULATION
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL DIFFERENTIATION
DATA
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
INFORMATION
INJURIES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NERVE CELLS
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURIFICATION
PYRIMIDINES
RADIOISOTOPES
RATS
RECEPTORS
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SCIATIC NERVE
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES