Plasmid-determined cadmium resistance in Pseuodomonas putida GAM-1 isolated from soil

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Bacteriol.; (United States)

OSTI ID:5801483

Horitsu, H; Yamamoto, K; Wachi, S; Kawai, K; Fukuchi, A

Cadmium-resistant pseudomonas putida GAM-1, which was able to grow in concentrations of CdCl/sub 2/ as high as 7 mM, was isolated from soil in a rice paddy. This bacterium harbored a DNA plasmid of about 52 kilobases. The plasmid (pGU100) transformed Escherichia coli C600 to cadmium resistance. A cadmium-resistant transformant of E. coli C600 contained a plasmid corresponding to that seen in P. putida GAM-1. The transformant did not take up cadmium as well as P. putida GAM-1 did. This is the first report on the mechanism of plasmid-determined Cd/sup 2 +/ resistance in gram-negative bacteria.

• Research Organization: Gifu Univ., Japan
• OSTI ID: 5801483
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 165:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700 -- Microbiology
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
CADMIUM
CELL CONSTITUENTS
DNA
ELEMENTS
ESCHERICHIA COLI
GROWTH
METALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
PSEUDOMONAS
SOILS
TOLERANCE
TOXICITY