Activation and increment of alveolar macrophages induced by nitrogen dioxide

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398609530819· OSTI ID:5800353

Mochitate, K; Takahashi, Y; Ohsumi, T; Miura, T

Male Wistar rats were exposed to 4 ppm nitrogen dioxide (NO/sub 2/) for 10 d, and at intervals alveolar macrophages were collected by pulmonary lavage. A metabolic enhancement of alveolar macrophages was observed on d 4 of exposure. The specific activities of glucose-6-phosphate dehydrogenase and glutathione peroxidase of the peroxidative metabolic pathway increased to 1.29-fold and 1.17-fold those of the control values, respectively. The specific activities of succinate-cytochrome c reductase of the mitochondrial respiratory system and pyruvate kinase of the glycolytic pathway also increased to 1.17-fold and 1.20-fold those of the control values, respectively. In addition, the incorporation of (/sup 3/H)leucine and (/sup 14/C)thymidine into alveolar macrophages were elevated to 1.77-fold and 1.84-fold those of the control values, respectively. The activities of all enzymes tested decreased to control levels by d 10. The number of alveolar macrophages collected from exposed animals increased to 1.24-fold that of the control value of d 7 and was maintained at a significantly higher level until d 10. Alveolar macrophages were heterogeneous in size (7-21 ..mu..m in diameter), and most of them were distributed between 11 and 17 ..mu..m in diameter. Exposures to 4 ppm NO/sub 2/ increased significantly the cells of 9-13 ..mu..m in diameter on the seventh day. These results show that exposures to 4 ppm NO/sub 2/ cause a metabolic enhancement and subsequent increase in alveolar macrophages.

• Research Organization: National Institute for Environmental Studies, Yatabe, Japan
• OSTI ID: 5800353
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 17:2-3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUGMENTATION
AZINES
BIOLOGICAL PATHWAYS
CARBOXYLIC ACIDS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
HETEROCYCLIC COMPOUNDS
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MACROPHAGES
MAMMALS
METABOLISM
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHAGOCYTES
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES