Increase in the activities of glycolytic enzymes in rat lungs produced by nitrogen dioxide

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398509530658· OSTI ID:5443225

Mochitate, K; Miura, T; Kubota, K

Male Jcl: Wistar rats were exposed to 2, 4, and 10 ppm NO/sub 2/ for 14, 10, and 7 d, respectively, to examine the effect of NO/sub 2/ on the lung glycolytic pathway, a major energy-generating system in the lung. A highly significant increase in the activities of hexokinase, phosphofructokinase, 3-phosphoglycerate kinase, pyruvate kinase (PK), and lactate dehydrogenase was observed after 5 d exposure to 10 ppm NO/sub 2/, and a significantly higher value was maintained until d 7. Similarly, the activities of all enzymes examined increased significantly by exposure to 4 ppm NO/sub 2/, reaching the maximum between 4 and 7 d of exposure, and then approached to near the control levels. The most remarkable increase was found in the PK activity, which reached 1.82- and 1.53-fold that of the control at d 5 (10 ppm) and d 7 (4 ppm) of exposure, respectively. Upon exposure to 2 ppm NO/sub 2/, the PK activity of exposed animals was also increased to 1.23-fold that of the control at d 7, and higher activity was maintained until d 14. The glucose-6-phosphate dehydrogenase activity of exposed animals increased significantly at d 3, 4, and 14 of exposures to 10, 4, and 2 ppm NO/sub 2/, respectively, and a significantly higher value was maintained in the following period of exposure. These results show that short-term exposure of rats to 2-10 ppm NO/sub 2/ induces the pulmonary systems concerning glycolysis and NADPH-generation. The generation of energy and NADPH in the lung may be enhanced by NO/sub 2/ inhalation.

• Research Organization: National Institute for Environmental Studies, Yatabe, Japan
• OSTI ID: 5443225
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 15:2; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL PATHWAYS
BODY
CHALCOGENIDES
CHEMICAL REACTIONS
COENZYMES
DATA
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GLYCOLYSIS
INFORMATION
INHALATION
INTAKE
LUNGS
MAMMALS
METABOLISM
NADP
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RATS
RESPIRATORY SYSTEM
RODENTS
TOXICITY
TRANSFERASES
VERTEBRATES