Biological (Molecular and Cellular) Markers of Toxicity: Semi-annual Technical Progress Report (No. 5). October 1, 1990, to March 30, 1991

Technical Report · Mon Apr 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5797375· OSTI ID:5797375

Shugart, Lee R. ^[1]

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1. Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

The objective of this study is to evaluate the use of the Japanese Medaka (Oryzias latipes) as a predictor of genotoxicity following exposure to carcinogens. The early molecular events associated with genotoxicity in Medaka tissues following exposure to known carcinogens will be investigated. The primary endpoint for most small fish carcinogenesis studies is histopathogenic identification of a neoplastic lesion. Such lesions usually occur in the liver, and histogenesis of liver neoplasms in fish is similar to that in rodents. Because of the latent period between initial contact with chemical agents in the environmental and subsequent expression of deleterious effects, development of sensitive assays for detection and estimating early exposure is needed. Carcinogen-induced DNA damage will be assessed as a possible measure of severity of exposure, correlated with activation of liver enzymes.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOD; USDOE
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5797375
• Report Number(s): ORNL/M--1426; ON: DE91013546
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ADDUCTS
AMINES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOLOGICAL MARKERS
BIOLOGICAL MODELS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL STRESS
BODY
CARCINOGEN SCREENING
CARCINOGENESIS
CARCINOGENS
CONDENSED AROMATICS
DETOXIFICATION
DIGESTIVE SYSTEM
DISEASES
DNA ADDUCTS
DNA REPAIR
DOCUMENT TYPES
ENVIRONMENTAL EXPOSURE
ENZYME ACTIVITY
FISHES
GENOME MUTATIONS
GLANDS
HYDROCARBONS
LIVER
MUTATIONS
NEOPLASMS
NITROSAMINES
NITROSO COMPOUNDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PROGRESS REPORT
PROTEINS
RECOVERY
REPAIR
SCREENING
STRAND BREAKS
TISSUES
TOXICITY
VERTEBRATES