Acetone and butanol production by Clostridium acetobutylicum in a synthetic medium

Journal Article · Wed Dec 01 04:00:00 EST 1982 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5792720

Monot, F; Martin, J R; Petitdemange, H; Gay, R

The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO/sub 4/, 50 to 200 mg/liter; MnSO/sub 4/, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH/sub 2/PO/sub 4/ and K/sub 2/HPO/sub 4/); FeSO/sub 4/, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter. (Refs. 24).

• Research Organization: Lab des Sciences du Genie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France; Univ de Nancy I, Lab de Chimie Biologique I, 54037 Nancy, Cedex, France
• OSTI ID: 5792720
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 44:6; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACETONE
ALCOHOLS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BUTANOLS
CHEMICAL REACTIONS
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
CULTURE MEDIA
DECOMPOSITION
GROWTH
HYDROLYSIS
HYDROXY COMPOUNDS
KETONES
KINETICS
LYSIS
METHANOGENIC BACTERIA
MICROORGANISMS
NUTRIENTS
ORGANIC COMPOUNDS
PRODUCTION
QUANTITY RATIO
REACTION KINETICS
SOLVOLYSIS
SYNTHESIS