Regulation and butanol inhibition of d-xylose and d-glucose uptake in Clostridium acetobutylicum

Journal Article · Mon Apr 01 04:00:00 EST 1985 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5351522

Ounine, K; Petitdemange, H; Raval, G; Gay, R

Clostridium acetobutylicum exhibited diauxic growth in the presence of mixtures of glucose and xylose. Both glucose- and xylose-grown cells had a glucose uptake activity. On the other hand, growth on xylose was associated with the induction of a xylose permease activity, which was repressed by glucose in xylose-induced cells. The rate of sugar uptake with increasing sugar concentrations showed saturation kinetics with an apparent K/sub m/ of 1.25 x 10/sup 5/ M for glucose and 5 x 10/sup 3/ M for xylose. Concomitant with the production of solvents, the activities of the glucose and xylose transport systems decreased. Among the main products of fermentation, butanol was shown to be a potent inhibitor of the growth of the organism and of the rate of sugar uptake as well as of sugar incorporation into cell materials. These inhibitory effects of butanol were more pronounced in xylose-grown cells than in glucose-grown cells. Butanol completely inhibited growth at a concentration of 14 g/liter for cultures growing on glucose and 8 g/liter for cultures growing on xylose. Concentrations of 7 and 10.5 g/liter of butanol caused a 50% inhibition of the xylose and glucose incorporations into cell materials. These inhibitory levels of butanol were found in typical glucose or xylose fermentations.

• Research Organization: Univ. Nancy 1, Cedex, France
• OSTI ID: 5351522
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 49:4; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BUTANOLS
CARBOHYDRATES
CLOSTRIDIUM
CLOSTRIDIUM ACETOBUTYLICUM
FERMENTATION
GLUCOSE
GROWTH
HEXOSES
HYDROXY COMPOUNDS
INHIBITION
KINETICS
METABOLISM
METHANOGENIC BACTERIA
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
REACTION KINETICS
SACCHARIDES
XYLOSE