Structure of long terminal repeats of transcriptionally active and inactive copies of Drosophila mobile dispersed genetic elements mdg3

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5785063

Dzhumagaliev, E B; Mazo, A N; Baev, Jr, A A; Gorelova, T V; Arkhipova, I R; Shuppe, N G; Il'in, Yu V

The authors have determined the nucleotide sequences of long terminal repeats (LTRS) and adjacent regions in the transcribed and nontranscribed variants of the mobile dispersed gene mdg3. In its main characteristics the mdg3 is similar to other mdg. Its integration into chromosomal DNA brings about duplication of the 4 bp of the host DNA, no specificity of the mdg integration at the nucleotide level being detected. The mdg3 is flanked by a 5 bp inverted repeat. The variations in the length of the LTR in different mdg copies is mainly due to duplication of certain sequences in the U3 and R regions. mdg3 copies with a LTR length of 267 bp are the most abundant and are completely conservative in their primary structure. They are transcribed in the cells of the 67J25D culture, but not transcribed in the K/sub c/ line, where another mdg3 variant with a LTR length of 293 bp is transcriptionally active. The SI mapping of transcription initiation and termination sites has shown that in both mdg3 variants they are localized in the same LTR regions, and that the LTR itself has a characteristic U3-R-U5 structure-like retroviral LTRs. The possible factors involved in the regulation of mdg transcription are discussed.

• Research Organization: Institute of Molecular Biology, Moscow, USSR
• OSTI ID: 5785063
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 22:3; ISSN SOGEB
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
ANIMALS
ARTHROPODS
BIOLOGY
CELL CULTURES
CHEMICAL REACTIONS
CHROMOSOMES
CLONING
DECOMPOSITION
DIPTERA
DNA REPLICATION
DNA SEQUENCING
DNA-CLONING
DROSOPHILA
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
FLIES
FRUIT FLIES
GENES
GENETIC MAPPING
GENETICS
HYDROLASES
HYDROLYSIS
INSECTS
INVERTEBRATES
LABELLED COMPOUNDS
LABELLING
LYSIS
MAPPING
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEASES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHATASES
PHOSPHODIESTERASES
PHOSPHORUS COMPOUNDS
RADIOENZYMATIC ASSAY
RNA
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION