Fine structure of long terminal repeats and the stages of reverse transcription of Drosophila mobile dispersed genes

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5785055

Mazo, A M; Arkhipova, I R; Cherkasova, V A; Gorelova, T V; Shuppe, N G; Il'in, Yu V

The authors detected in Drosophila melanogaster cultured cells poly(A)-containing intermediate forms of reverse transcription of the RNA of mobile dispersed genes mdgl, mdg3, and mdg4, associated with the initiation of the synthesis of minus and plus chains of the DNA (minus and plus strong-stop DNA). A comparative analysis of intermediates revealed that reverse transcription of mdg passes through the same stages as in retroviruses, showing complete similarity between the intermediate forms, and that despite some differences in their structure all mdg investigated possess a common mechanism of reverse transcription. The length of the minus strong-stop DNA, giving the position of the point of initiation of RNA synthesis, is in agreement with data obtained from the SI analysis on initiation of mdg transcription. Using SI analysis, they have shown for the first time that the mdg RNA contains at its termini a repeated region R, similarly as in retroviral RNA, and that the long terminal repeats of mdg have a U3-R-U5 structure similar to that of proretroviral LTRs. Initiation of transcription for all three mdg occurs within or immediately after the same TCAGTPy sequence, it not being possible to detect sequences resembling the TATA and CAAT boxes in the 5' nontranscribed mdg region.

• Research Organization: Institute of Molecular Biology, Moscow, USSR
• OSTI ID: 5785055
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 22:3; ISSN SOGEB
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BIOSYNTHESIS
CELL CULTURES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIPTERA
DNA
DNA POLYMERASES
DNA REPLICATION
DNA SEQUENCING
DROSOPHILA
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
ESTERASES
FLIES
FRUIT FLIES
GENES
GENETICS
HYDROLASES
HYDROLYSIS
INSECTS
INVERTEBRATES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSIS
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEASES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSCRIPTION
TRANSFERASES
VIRUSES