Expression of phage-transposons of Pseudomonas aeruginosa in cells of Pseudomonas putida PpGl. II. Zygotic induction, an essential condition for the emergence of defective lysogens
The presence of a large number of clones, which have lost the ability to produce phase, is a feature of PpGl exconjugants, which have acquired markers of the hybrid plasmid containing the genome of the PAO1 phage transposon. Analysis of these clones indicates that they contain plasmids with different defects in the phage genome (point mutations and different deletion lengths which may have an effect on both the phage genome and also plasmid RP4). Mutations of a particular region are selected, the region of early prophage genes. The process of zygotic induction is an essential condition for the arisal of mutants (including deletion mutants). The results of the experiment, which was based on the Luria-Delbruik test, showed that a significant proportion of the mutations, including deletion mutations, arise in the recipient cells PpGl.
- Research Organization:
- All-Union Scientific-Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR
- OSTI ID:
- 5785042
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 21:12; Other Information: Translated from Genetika; 21: No. 12, 1937-1944(Dec 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIOPHAGES
BIOSYNTHESIS
CLONE CELLS
CHROMOSOMAL ABERRATIONS
GENE MUTATIONS
GENOME MUTATIONS
TRACER TECHNIQUES
PSEUDOMONAS
GENETICS
LYSIS
CHROMOSOMES
CROSSING-OVER
DNA
HYBRIDIZATION
LABELLED COMPOUNDS
LABELLING
MUTAGENESIS
MUTANTS
PHOSPHORUS 32
PLASMIDS
TRANSCRIPTION
ZYGOTES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
CELL CONSTITUENTS
CELL CULTURES
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SYNTHESIS
VIRUSES
550401* - Genetics- Tracer Techniques