Hepatic handling of bile salts and protein in the rat during intrahepatic cholestasis
17 alpha-Ethynyl estradiol-induced cholestasis was used to study the relationship of protein to bile salt transport in liver. The biliary secretion of horseradish peroxidase was unaltered in treated animals despite a 56% reduction in bile flow. Cytochemistry confirmed that estradiol caused no alteration in the handling of tracer. In a second study, the peak biliary secretion of (/sup 14/C)taurocholate was reduced by approximately 46% in treated animals. The kinetics of /sup 125/I-cholyglycylhistamine, a bile salt derivative, were identical to those of taurocholate in control and cholestatic animals. Taurocholate and cholylglycylhistamine secretion were markedly reduced in control animals during competition with unlabeled taurocholate. Quantitative electron microscopic autoradiography with /sup 125/I-cholylglycylhistamine revealed a high concentration of grains over the endoplasmic reticulum and the Golgi complex including associated lysosomes and vesicles. These data demonstrate that estradiol markedly inhibits bile salt transport, but not vesicular transport of horseradish peroxidase. Furthermore, estradiol may alter the movement of bile salts through these organelles.
- Research Organization:
- Cell Biology and Aging Section, Veterans Administration Medical Center, San Francisco, California
- OSTI ID:
- 5776794
- Journal Information:
- Gastroenterology; (United States), Journal Name: Gastroenterology; (United States) Vol. 84:5; ISSN GASTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BILE
BILIARY TRACT
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTRADIOL
ESTRANES
ESTROGENS
FUNCTIONS
GLANDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHYSIOLOGY
PORPHYRINS
PROTEINS
RADIOISOTOPES
SECRETION
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES