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Characterization of calcium deprivation-induced cholestasis in the perfused rat liver

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5151104
; ; ;

To elucidate the role of calcium in regulation of canalicular bile flow, the authors studied biliary sucrose permeability and the transport kinetics of taurocholate in the in situ perfused rat liver. Calcium deprivation did not adversely affect viability or ultrastructural appearances of the liver. Removal of calcium led to initial choleresis followed by cholestasis dependent on external ionized calcium concentration. Biliary recovery of (/sup 14/C)sucrose relative to that of tritiated water was determined by a biliary multiple-indicator dilution technique. Analysis in terms of irreversible thermodynamics suggested that biliary permeability to sucrose increased due to a change in the sieving coefficient from 0.135 to 0.435. Biliary recovery of taurocholate was significantly (P less than 0.001) reduced in low-calcium medium (from 79.6 +/- 6.5 to 17.6 +/- 11.8%). This was not due to a defect in hepatocellular uptake of taurocholate as determined in the perfused rat liver by a multiple-indicator dilution technique and in isolated hepatocytes. The authors conclude that calcium deprivation-induced cholestasis is characterized by an increased biliary permeability, a defect in cellular translocation and/or canalicular secretion of bile salts, and a defect in bile salt-independent bile flow.

Research Organization:
Univ. of Colorado Medical School, Denver
OSTI ID:
5151104
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
BILE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CALCIUM
CARBOHYDRATES
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
DISACCHARIDES
ELEMENTS
GLANDS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METALS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PERFUSED ORGANS
PERMEABILITY
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SACCHAROSE
SULFONIC ACIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES