Transplantation of islet cells across major histocompatibility barriers after total lymphoid irradiation and infusion of allogeneic bone marrow cells
Diabetic Lewis rats (AgB1/L) were evaluated as recipients of allogeneic Wistar-Furth (AgB2/2) isolated adult islets without the use of standard recipient immunosuppression. One group was treated with fractionated total lymphoid irradiation (TLI) and Wistar-Furth bone marrow cell reconstitution to proven chimerism prior to islet transplantation. This group returned to a prediabetic state following Wistar-Furth islet transplantation without any evidence of rejection for 100 days posttransplant. A second group of Lewis rats received only TLI without bone marrow treatment. They gave a varying result following islet transplantation with one recipient showing evidence of prolonged islet survival. A third chimeric control group did not receive isolated islets and did not alter their diabetic state. A fourth group was not given TLI nor donor bone marrow cells and uniformly rejected their allogeneic islets by 7 days. Thus, allogeneic adult islets will survive across major rat histocompatibility barriers using TLI and donor bone marrow chimerism as the only form of immunosuppression.
- Research Organization:
- Washington University School of Medicine, Department of Surgery, St. Louis, Missouri
- OSTI ID:
- 5776066
- Journal Information:
- Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 31 Suppl 4; ISSN DIAEA
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BODY
BONE MARROW CELLS
CHIMERAS
CONNECTIVE TISSUE CELLS
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
FRACTIONATED IRRADIATION
GLANDS
GRAFT-HOST REACTION
IMMUNOSUPPRESSION
IRRADIATION
LYMPHATIC SYSTEM
MAMMALS
METABOLIC DISEASES
MOSAICISM
ORGANS
PANCREAS
RADIATION CHIMERAS
RATS
RODENTS
SOMATIC CELLS
TRANSPLANTS
VERTEBRATES