DNA repair defects associated with chromosomal translocation breaksite regions

Journal Article · Tue Feb 01 04:00:00 EST 1994 · Molecular and Cellular Biology

DOI:https://doi.org/10.1128/MCB.14.2.1204· OSTI ID:577133

Beecham, E J; Link, C; Bohr, V A ^[1]

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1. National Cancer Inst., Bethesda, MD (United States); and others

Using an assay that measures the removal of UV-induced pyrimidine dimers in specific DNA sequences, we have found that the Pvt-1, immunoglobulin H-C{alpha} (IgH-C{alpha}), and IgL-{kappa} loci are poorly repaired in normal B lymphoblasts from plasmacytoma-susceptible BALB/cAnPt mice. Breaksites in these genes are associated with the chromosomal translocations that are found in >95% of BALB/cAnPt plasmacytomas. In contrast to those from BALB/cAnPt mice, B lymphoblasts from plasmacytoma-resistant DBA/2N mice rapidly repair Pvt-1, IgH-C{alpha} and IgL-{kappa}. Further, (BALB/cAnPt x DBA/2N)F{sub 1} hybrids, which are resistant to plasmacytoma development, carry an efficient (DBA/2N-like) repair phenotype. Analysis of allele-specific repair in the IgH-C{alpha} locus indicates that efficient repair is controlled by dominant, transacting factors. In the F{sub 1} heterozygotes, these factors promote efficient repair of BALB/cAnPt IgH-C{alpha} gene sequences. The same sequences are poorly repaired in the BALB/cAnPt parental strain. Analysis of the strand specificity of repair indicates that both strand-selective and nonselective forms of repair determine repair efficiency at the gene level in nonimmortalized murine B lymphoblasts. 36 refs., 3 figs., 6 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 577133
• Journal Information: Molecular and Cellular Biology, Journal Name: Molecular and Cellular Biology Journal Issue: 2 Vol. 14; ISSN 0270-7306; ISSN MCEBD4
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA
DNA HYBRIDIZATION
DNA REPAIR
DNA SEQUENCING
EFFICIENCY
ELECTROPHORESIS
GENE MUTATIONS
GENES
GENETIC VARIABILITY
IMMUNOGLOBULINS
LYMPHOMAS
MICE
NEOPLASMS
PHENOTYPE
PYRIMIDINE DIMERS
SPECIFICITY
ULTRAVIOLET RADIATION