DNA repair in the c-myc proto-oncogene locus: Possible involvement in susceptibility or resistance to plasmacytoma induction in BALB/c mice

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.6.3095· OSTI ID:5506839

Beecham, E J; Mushinski, J F; Shacter, E; Potter, M; Bohr, V A ^[1]

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1. Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, MD (USA)

This report describes an unexpected difference in the efficiency of removal of UV-induced DNA damage in the c-myc locus in splenic B lymphoblasts from two inbred strains of mice. In cells from plasmacytoma-resistant DBA/2N mice, 35% of UV-induced damage in the regulatory and 5{prime} flank of c-myc is removed by 12 h. However, in cells from plasmacytoma-susceptible BALB/cAn mice, damage is not removed from this region. In the protein-encoding region and 3{prime} flank of c-myc as well as in two dihydrofolate reductase gene fragments, UV damage is repaired with similar efficiency in B lymphoblasts from both strains of mice. Furthermore, in the protein-encoding portion and 3{prime} flank of c-myc, damage is selectively removed from only the transcribed strand. No repair is detected in the nontranscribed strand. In contrast, DNA repair in the 5{prime} flank of c-myc is not strand specific; in DNA from DBA/2N cells, UV damage is rapidly removed from both the transcribed and nontranscribed strands. In BALB/cAn cells no repair was detected in either strand in the 5'flank, consistent with the results with double-stranded, nick-translated probes to this region of c-myc. In addition to the repair studies, we have detected post-UV-damage formation: in most of the genes studied, we find that additional T4 endonuclease-sensitive sites are formed in the DNA 2 h after irradiation. Our findings provide new insights into the details of gene-specific and strand-specific DNA repair and suggest that there may be close links between DNA repair and B-cell neoplastic development.

• OSTI ID: 5506839
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 11:6; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

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560120* -- Radiation Effects on Biochemicals
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
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ENDONUCLEASES
ENZYMES
ESTERASES
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYDROLASES
IMMUNITY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
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MICE
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ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANS
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