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Large introns in the 3' end of the gene for the pro. cap alpha. 1(IV) chain of human basement membrane collagen

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Using a recently characterized cDNA clone (HT-21) coding for the pro..cap alpha..1(IV) chain of human type IV procollagen, the authors have isolated three clones from a bacteriophage lambda Charon 4A library of human genomic DNA. The intron/exon structure of the pro..cap alpha..1(IV) genomic clones was analyzed by heteroduplex electron microscopy and nucleotide sequencing. The analysis showed that the introns separating exons 2-9 are large and have a total length of over 12,000 base pairs (bp). Six of seven exons at the 3' end of the gene coded for -Gly-Xaa-Yaa-repeats of the collagenous part of the chain. Five of the -Gly-Xaa-Yaa-coding exons (numbers 5-9) varied in size between 72 bp and 134 bp, and none of them were 54 bp or multiples thereof. A sixth exon (exon 4) was a junction exon containing 71 bp coding for-Gly-Xaa-Yaa-sequences and 142 bp coding for the carboxyl-terminal noncollagenous domain (NC-1). The seventh exon (exon 3, 178 bp) coded for sequences of the NC-1 domain. Five of the six-Gly-Xaa-Yaa- coding exons began with the second base coding for glycine, and only one exon began with a complete glycine codon at the 5' end. The results (i) suggest that the gene for the pro..cap alpha..1(IV) chain of human basement membrane collagen is significantly larger than the genes for fibrillar collagens and (ii) show that it lacks the 54-bp exon repeats characteristic of fibrillar collagen genes.

Research Organization:
Univ. of Medicine and Dentistry of New Jersey-Rutgers Medical School, Piscataway
OSTI ID:
5767968
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:6; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
COLLAGEN
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTRON MICROSCOPY
EVEN-ODD NUCLEI
GENES
GENETIC MAPPING
GENETICS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MICROORGANISMS
MICROSCOPY
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SCLEROPROTEINS
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES
VIRUSES